mouse anti 5 ht2a  (Santa Cruz Biotechnology)

Journal: Acupuncture in Medicine

Article Title: Electroacupuncture alleviates acute myocardial ischemic injury in mice by regulating the β 1 adrenergic receptor and post-receptor protein kinase A signaling pathway

doi: 10.1177/09645284241298716

Figure Lengend Snippet: Effects of electroacupuncture (EA) on the level of norepinephrine (NE), post-receptor protein kinase A (PKA) signaling pathway and expression levels of L-type voltage-gated calcium channel α1 C (Cav1.2), serine phosphorylated 16-phospholamban (p-PLB s16 ) and sarcoplasmic reticulum (SR) Ca 2+ -adenosine triphosphate (ATP)ase 2a (SERCA2a) in myocardial tissues in vivo . (a) NE levels in myocardial tissue (n = 3 per group). (b) Representative Western blot images of stimulatory G protein (Gs), phosphorylated PKA (p-PKA), Cav1.2, p-PLB s16 and SERCA2a. (c–g) Quantification of Gs, p-PKA, Cav1.2 p-PLB s16 and SERCA2a protein expression levels (n = 3 per group). (h, i) Levels of adenylate cyclase (AC) and cyclic adenosine monophosphate (cAMP) in myocardial tissue (n = 3 per group). Data are mean ± SD. * p < 0.05, ** p < 0.01.

Article Snippet: After that, the film was transferred with 5% skim milk powder and then incubated with the following primary antibodies in the refrigerator overnight at 4°C: anti-β 1 (1:1000; Ab8226, Abcam, Cambridge, UK); anti-Gs (1:500; Ab235956, Abcam); anti-phosphorylated (p)-PKA (1:2500; Ab75991, Abcam); anti-Cav1.2 (1:500; ACC-003, Alomone Labs, Israel); anti-p-phospholamban (PLB; 1:10,000; Ab92697, Abcam); anti-sarcoplasmic reticulum (anti-SR) Ca 2+ -ATPase 2a (SERCA2a; 1:10,000; A010–23S, Badrilla, China); anti-cleaved caspase 3 (1:500; Ab32042, Abcam); and anti-β-actin (1:1000; Ab8226, Abcam).

Techniques: Expressing, In Vivo, Western Blot

Journal: Acupuncture in Medicine

Article Title: Electroacupuncture alleviates acute myocardial ischemic injury in mice by regulating the β 1 adrenergic receptor and post-receptor protein kinase A signaling pathway

doi: 10.1177/09645284241298716

Figure Lengend Snippet: Schematic representation of the cardiac β 1 adrenergic receptor (β 1 -AR) and post-receptor protein kinase A (PKA) signaling pathway regulated by electroacupuncture (EA). MI: myocardial ischemia; LAD: left anterior descending coronary artery; NE: norepinephrine; Gs: stimulatory G protein; AC: adenylate cyclase; cAMP: cyclic adenosine monophosphate; Cav1.2: L-type voltage-gated calcium channel α1 C; p-PLB s16 : serine phosphate 16-phospholamban; SERCA2a: sarcoplasmic reticulum Ca 2+ -adenosine triphosphate (ATP)ase 2a.

Article Snippet: After that, the film was transferred with 5% skim milk powder and then incubated with the following primary antibodies in the refrigerator overnight at 4°C: anti-β 1 (1:1000; Ab8226, Abcam, Cambridge, UK); anti-Gs (1:500; Ab235956, Abcam); anti-phosphorylated (p)-PKA (1:2500; Ab75991, Abcam); anti-Cav1.2 (1:500; ACC-003, Alomone Labs, Israel); anti-p-phospholamban (PLB; 1:10,000; Ab92697, Abcam); anti-sarcoplasmic reticulum (anti-SR) Ca 2+ -ATPase 2a (SERCA2a; 1:10,000; A010–23S, Badrilla, China); anti-cleaved caspase 3 (1:500; Ab32042, Abcam); and anti-β-actin (1:1000; Ab8226, Abcam).

Techniques:

Journal: Frontiers in Pharmacology

Article Title: Bmal1 haploinsufficiency impairs fear memory and modulates neuroinflammation via the 5-HT2C receptor

doi: 10.3389/fphar.2024.1422693

Figure Lengend Snippet: LPS reversed the impairment of cued fear learning and memory in Bmal1 +/− mice. (A) Representative Western blot images and protein levels of Bmal1 in the PFC of Bmal1 +/+ and Bmal1 +/− mice after LPS injection. n = 6 mice. Data are reported as mean ± SEM. Unpaired t-test. ns, no significance; *p < 0.05; **p < 0.01. (B) Schematic representation of the experimental timeline. LPS was administrated 7 days before fear conditioning. n = 4 mice. (C, D) Freezing levels of Bmal1 +/− mice in cued fear conditioning and recall test. Bmal1 +/− group, n = 6 mice; Bmal1 +/− + LPS group, n = 6 mice. Data are reported as mean ± SEM. Unpaired t-test. Bmal1 +/− group vs. Bmal1 +/− + LPS group. ns, no significance; *p < 0.05; **p < 0.01. (E) Representative Western blot images and protein levels of 5-HT2CR, synaptophysin, PSD95, synapsin-1, SNAP25, p-PKA, and p-PKC in the PFC of Bmal1 +/− mice after LPS injection (D) . n = 6 mice. Data are reported as mean ± SEM. Unpaired t-test. Bmal1 +/− group vs. Bmal1 +/− + LPS group. ns, no significance; *p < 0.05; **p < 0.01.

Article Snippet: 5-HT2CR , Santa Cruz , SC-166775 , 1:500.

Techniques: Western Blot, Injection

Journal: Frontiers in Pharmacology

Article Title: Bmal1 haploinsufficiency impairs fear memory and modulates neuroinflammation via the 5-HT2C receptor

doi: 10.3389/fphar.2024.1422693

Figure Lengend Snippet: Detailed antibody information.

Article Snippet: 5-HT2CR , Santa Cruz , SC-166775 , 1:500.

Techniques:

Journal: Frontiers in Pharmacology

Article Title: Bmal1 haploinsufficiency impairs fear memory and modulates neuroinflammation via the 5-HT2C receptor

doi: 10.3389/fphar.2024.1422693

Figure Lengend Snippet: Bmal1 +/− mice show abnormal neurotransmitter levels and molecular signals. (A–F) Levels of neurotransmitters in the PFC of Bmal1 +/+ and Bmal1 +/− mice after cued fear conditioning paradigm. 5-HT (A) , dopamine (B) , GABA (C) , glutamate (D) , norepinephrine (E) , and acetylcholine (F) . n = 7 mice. Data are reported as mean ± SEM. Unpaired t-test. ns, no significance; *p < 0.05; **p < 0.01. (G, H) Representative Western blot images (G) and protein levels of IDO, 5-HT2CR, SIRT1, TPH2, TH, DAT, p-IP3, p-PKC, and p-CREB in the PFC of Bmal1 +/+ and Bmal1 +/− mice after the cued fear conditioning paradigm (H) . n = 4–6 mice. Data are reported as mean ± SEM. Unpaired t-test. ns, no significance; *p < 0.05; **p < 0.01.

Article Snippet: 5-HT2CR , Santa Cruz , SC-166775 , 1:500.

Techniques: Western Blot